2024 Cleaved atf6 antibody

Cleaved atf6 antibody

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August undefined, 2024

WebUnder ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. . Information by UniProt ... All lanes : Anti-ATF6 antibody (ab134561) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 … WebThe ATF6 band may represent under glycosylated or cleaved/active ATF6. Western blot analysis of SM7007P ATF6 antibody. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells.

ATF-6α Antibody (F-7) SCBT - Santa Cruz Biotechnology

Web221 rows · ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an … WebATF6 Antibodies. Antibodies that detect ATF6 can be used in several scientific applications, including Western Blot, Immunohistochemistry, … marc raziano

ATF6 Mediates a Pro-Inflammatory Synergy Between ER Stress …

WebATF6 band may represent under glycosylated or cleaved/active ATF6. Western blot analysis of SM7007P ATF6 antibody. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells. Western blots were probed with 4 ug/ml of the monoclonal … WebUsing this system, the functional domain of cleaved ATF6 is fused to ERT2. In the absence of ERT2 ligands (e.g., tamoxifen), ERT2 forms a complex with various cellular proteins, such as Hsp90, which mask ATF6. The addition of tamoxifen, however, blocks Hsp90 binding to ERT2, allowing ERT2–ATF6 to induce transcriptional activity of ATF6. WebATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen, 2005 & Prywes, 2005). c\u0026h precision

ATF6 (Full-length and Active / Cleaved Forms) Antibody …

ATF6 antibody (24169-1-AP) Proteintech - ptglab

WebATF6 is a type II transmembrane protein, with a bZIP transcription factor domain in its cytosolic domain. During ER stress, ATF6 is released from Grp78 binding, and traffics to the Golgi, where it is cleaved by site-1 and site-2 proteases (S1P and S2P) (Shen et al., 2002; Ye et al., 2000). WebProteintech Anti-ATF6 Polyclonal, Catalog # 24169-1-AP. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry … c\\u0026h international air consolidatorWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-ATF6 antibody [EPR22690-84] - ChIP Grade (ab227830) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment ... marcrebillet

"WebDec 1, 2000 · When unfolded proteins accumulate in the ER, ATF6 is cleaved proteolytically to release the NH 2-terminal domain, which enters the nucleus (Haze et al. 1999). ... To test this hypothesis, we used an antibody against GRP78, an ER chaperone that is known to be induced directly by ATF6 (Haze et al. 1999). " - Cleaved atf6 antibody

Cleaved atf6 antibody

Phospho-ATF6 (Thr166) Polyclonal Antibody - Thermo Fisher Scientific

WebBased on 100% sequence alignment, this antibody detects ATF6 isoform A (alpha). The antibody is predicted to recognize the 50kDa fragment as it recognizes a peptide … WebDec 27, 2016 · The cleaved ATF6 fragment migrates to the nucleus to transcriptionally up-regulate protein-folding enzymes and chaperones. ATF6 mutations in patients with achromatopsia include missense, nonsense, splice site, and single-nucleotide deletion or duplication changes found across the entire gene.

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WebFeb 15, 2016 · Herein, we report that ATF6 and its cleaved N-terminal cytoplasmic domain (known as ATF6a) are expressed in growth plate chondrocytes. We find that these proteins are differentially induced during BMP2-triggered chondrocyte differentiation. This differential expression probably results from the activation of the ATF6 gene by Runx2 and its ... WebApr 14, 2024 · Similarly western blot showed higher levels of cleaved ATF6 consistent with its activation in MPI KO cells ... Samples were then incubated with secondary antibody (AlexFluor 488, goat anti-rabbit ...

WebCompare Anti-ATF6 alpha (full length Cleaved Forms) Anti-ATF6 alpha (full length + Cleaved Forms) Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Webthe nuclear form of ATF6 (ATF6 p50), respectively. Expression levels of BiP and GRP94 were determined by Western blot analysis by using a muine anti-BiP monoclonal antibody and a murine anti-GRP94 monoclonal antibody, respectively. The membranes were re-blotted with an anti-α-actin antibody for control of sample loading. D Vecto r p

WebThe results show that DOM-1 increased the cleavage of ATF6 protein, increased the phosphorylation of EIF2AK3, and increased the abundance of cleaved XBP1 mRNA. Activation of these pathways led to an increased abundance of mRNA of the ER stress target genes GRP78, GRP94, and CHOP. Although CHOP is widely associated with … Web(ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa …

WebJan 31, 2024 · The ATF6 pathway is presumably activated during viral infection, as suggested by the proteolytic cleavage of p90ATF6, whereas the production of the active p50ATF6 fragment and the activation of its downstream target gene BiP in an ATF6- and ERSE-dependent manner do not occur [ 7 ].

WebApr 12, 2024 · Cleaved ATF6 also translocates to the nucleus and together with XBP1 enhances through transcriptional activation, ... Each membrane was blocked for 2 h in 5% milk diluted in 1× PBS, 0.1% Tween 20. Primary antibody incubation was performed overnight at 4°C in 1× PBS, 0.1% Triton X-100, 1% BSA, 0.02% azide. ... c \u0026 h polar/nonpolar/ionicWebCleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription ... anti-ATF6 antibody (AA 431-530) - Product Details Author: antibodies-online Inc. Subject: ATF6 antibody (ABIN4996973). Validated for ELISA, ICC, … marc record definitionWebCD51 + CD61 (Integrin alpha V + Integrin beta 3) CD51 / Integrin alpha V. CD52 c \u0026 i full formWebMar 17, 2024 · ATF-6α Antibody (F-7) is a mouse monoclonal IgG 1 κ ATF-6α antibody, cited in 139 publications, provided at 200 µg/ml raised against amino acids 31-310 of ATF-6 of human origin c \u0026 k collision carrollton ilWebWhen unfolded proteins accumulate in the ER, ATF6 is cleaved to release its cytoplasmic domain, which enters the nucleus. Here, we show that ATF6 is processed by Site-1 protease (S1P) and Site-2 protease (S2P), the enzymes that process SREBPs in response to cholesterol deprivation. marc rebillet nzWebUsing this system, the functional domain of cleaved ATF6 is fused to ERT2. In the absence of ERT2 ligands (e.g., tamoxifen), ERT2 forms a complex with various cellular proteins, … c \u0026 h tool peoria ilWebNov 25, 2015 · Treatment with UP resulted in reduced increment of ATF6 and CHOP, and recovered the decrease of phosphorylation of Akt/mTOR by tunicamycin and the increment of autophagy. ... such as cleaved-PARP and cleaved-Caspase-3. ... (Ser473) antibody (Cell Signaling Technology, Danvers, MA, USA); rabbit polyclonal anti-Akt antibody (Cell … c \\u0026 k collision carrollton il