2024 Mouse hepatic stellate cell line

Mouse hepatic stellate cell line

Author: emyj

August undefined, 2024

Nettet23. nov. 2024 · Fate-Mapping Evidence That Hepatic Stellate Cells Are Epithelial Progenitors in Adult Mouse Livers. Stem Cells. 26, 2104–2113 (2008). Article CAS PubMed PubMed Central Google Scholar DeLeve, L ... NettetMethods: Mouse LPCs were co-cultured in Transwell plates with an immortalized human hepatic stellate cell line (HSC-Li) we previously established. The morphology, …

Mouse Hepatic Stellate Cells - Innoprot Liver Cell System

Nettet22. jan. 2024 · Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells that reside within the subendothelial space of Disse around the liver sinusoid [ 1–3 ]. In healthy conditions, HSCs remain in the quiescent form and function primarily as fat-storing cell representing the largest reservoir of vitamin A (vitA) in the body [ 4 ]. Nettet26. mai 2024 · Primary hepatic stellate cells from two mice of the same group were pooled as one sample. The purity of primary HSCs was determined by adding Oil Red O staining to freshly isolated primary cells that had been cultured for 2 days (>97%). 24 HSC viability was determined using the Trypan blue exclusion test (>90%). scratchpad\\u0027s rc

Catechin-7-O-β-d-Apiofuranoside Inhibits Hepatic Stellate Cell ...

NettetNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum ... liver fibrosis. Since HSCs are major sources of fibrogenic liver cells, it was expected that Daxx would be expressed in hepatic stellate cells; however, the IHC data indicated that Daxx was mainly expressed in ... NettetHepatic stellate (HS) cells were isolated from the livers of metallothionein (MT)-null and control mice and used to establish IMS/MT(-) and IMS/N cell lines, respectively, using … NettetHepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCsHepatic stellate cells ... Phase contrast image of day 3 … scratchpad\\u0027s rn

Frontiers Targeting Hepatic Stellate Cells for the Treatment of …

Isolation and culture of hepatic stellate cells from mouse liver

NettetLX-2 Human Hepatic Stellate Cell Line, The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them a highly suitable model of human hepatic fibrosis. Expand SCC066 Nettet19. jun. 2024 · Hepatic stellate cell line LX-2 were obtained from the Cell Center of Shanghai Institutes for Biological Sciences. Although, the study of stellate cell … scratchpad\\u0027s rlNettet19. apr. 2024 · Hepatic stellate cells (HSCs) are specialized pericyte-like cells, located in the space of Disse, that wrap liver sinusoids [ 1] and interact with both hepatocytes and liver sinusoidal endothelial cells [ 2 … scratchpad\\u0027s ro

"Nettet11. apr. 2024 · During chronic hepatitis B virus (HBV) infection, hepatic fibrosis is a serious pathological condition caused by virus-induced liver damage. The activation of … " - Mouse hepatic stellate cell line

Mouse hepatic stellate cell line

Characterization of an immortalized hepatic stellate cell line ...

Nettet4. des. 2016 · High yield and high purity isolation of hepatic stellate cells from normal and fibrotic mouse liver - Co 1.77 MB Hepatic Stellate Cells Hematopoietic Stem Cells Stem Cell... Nettet11. mai 2024 · We first transfected Hepa 1–6 cells, a mouse hepatoma cell line, ... Hepatic stellate cells (HSCs) and Kupffer cells (KCs) were isolated according to the published protocol 29.

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Nettet12. okt. 2024 · Chu, P. S. et al. C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice. Hepatology 58 , 337–350 (2013). CAS PubMed Google Scholar Nettet19. apr. 2024 · To obtain meaningful results of hepatic stellate cell (HSC) function, it is crucial to use highly pure HSC populations. Our aim was to optimize HSC isolation from mice livers without exploiting the characteristically transient vitamin A autofluorescence of HSC. HSCs were isolated from C57BL/6 mice using a two-step collagenase digestion …

Nettet26. mai 2016 · Objective: Our aim was to establish a correlation between hepatic stellate cells (HSCs) activity within different areas of hepatic tissue and the degree of liver fibrosis,... NettetSome of the widely used hepatic cell lines are HepG2, Huh7, Hep3B, and SK-Hep-1, which are derived from hepatocellular carcinoma (HCC), and HepaRG, which is an HCC cell line that includes both ...

NettetMethods: Experiments were performed in wild-type and FNDC5-/-mice, primary mouse HSCs, and human hepatic stellate cell line (LX2). Mice were treated with carbon tetrachloride (CCl 4) or bile duct ligation (BDL) to induce liver fibrosis. EVs derived from HSCs were purified and injected intraperitoneally into mice. Nettet29. mai 2024 · Immortalized hepatic stellate cells (HSCs) established from mouse, rat, and humans are valuable in vitro models for the biomedical investigation of liver biology. These cell lines are homogenous, thereby providing consistent and reproducible results. They grow more robustly than primary HSCs and provide an unlimited supply of …

Nettet3. jan. 2014 · 16 The primary Kupffer cells and LSECs were isolated from mouse liver using liberase-based perfusion technique, low-speed centrifugation and magnetic-activated cell sorting (MACS). 17 Isolated ...

NettetC57BL/6 Mouse Primary Hepatic Stellate Cells from Cell Biologics are isolated from liver tissues of pathogen-free laboratory adult mice. Cells are grown in tissue culture flasks … scratchpad\\u0027s ruNettet4. okt. 2024 · Mouse hepatocyte cell line AML12 was purchased from the Chinese Academy of Sciences (Shanghai, China) and cultured in AML12 specific culture medium (Procell, Wuhan, China). Mouse hepatic stellate cell (CP-M041) was purchased from Procell (Wuhan, China) and maintained in specific hepatic stellate cell medium (CM … scratchpad\\u0027s rpNettet23. jul. 2015 · Intrahepatic implantation of mouse HCC cell lines requires 30 min per mouse. ... In addition, staining for the hepatic stellate cell activation markers α-SMA, ... scratchpad\\u0027s rsNettet29. mai 2024 · Immortalized hepatic stellate cells (HSCs) established from mouse, rat, and humans are valuable in vitro models for the biomedical investigation of liver … scratchpad\\u0027s rmNettetSCC064 Sigma-Aldrich LX-2 Human Hepatic Stellate Cell Line Download Zoom The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogen More>> scratchpad\\u0027s rrNettet11. mai 2024 · We first transfected Hepa 1–6 cells, a mouse hepatoma cell line, ... Hepatic stellate cells (HSCs) and Kupffer cells (KCs) were isolated according to the … scratchpad\\u0027s rvNettetDamage in Mice Juan E. Puche,1,2 Youngmin A. Lee,1 Jingjing Jiao,1 Costica Aloman,1 Maria I. Fiel,1 Ursula Mu~noz, 1,2 Thomas Kraus,4 Tingfang Lee,1 Hal F. Yee, Jr.,3 … scratchpad\\u0027s rw