Web13 okt. 2024 · Here we present our custom-designed miniscope GRadient INdex (GRIN) lens system that enables simultaneously recording from hundreds of neurons for months. This article includes miniscope design, … Web8 okt. 2024 · The preparation procedure consists of three steps, including (1) stereotaxically injecting the virus at the desired brain region of a mouse brain to label a specific …
Calcium imaging in freely moving mice during electrical …
WebDetailed schematic of the GRIN-lens-integrated nerve cuff for two-photon imaging and holographic stimulation. (A) GRIN-cuff situated on the cervical vagus nerve between the … WebAchieve immediate imaging results with our fully packaged solution for calcium imaging, which includes reagents, lenses, and validated protocols for brain-region specific … muddy mayhem knebworth
What Systems are Available for In Vivo Calcium …
Web24 jan. 2024 · calcium imaging. The CHEndoscope uses an implanted gradient index (GRIN) lens along with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice. This system is affordable, open-source, and flexible, permitting modification depending on the … WebGRIN lenses differ in lengths, enabling neuroscientists to image shallow to deep brain regions (up to 8mm). GRIN lenses are typically limited in diameter (0.5–1mm). Thus, imaging with GRIN lenses provides a relatively small field of view, compared to cortical windows, and the implantation of a large GRIN lens can lead to tissue damage, … Web23 aug. 2024 · In rodents, microendoscopic calcium imaging, which implants a gradient index (GRIN) lens into the brain and observes neural activity through a miniaturized fluorescent microscope, is also widely ... muddy memories