Orfh79
Witryna30 kwi 2015 · The DNA sequence analysis results suggest no homologous sequence exists between L-sp1 and orfH79 or atp6-orfH79. In wild rice, 19 of 102 investigated wild rice accessions could amplify PCR products of ~240 bp when using orfH79 sequence-specific primers O1 and O2, a similar product was amplified from YTA. Witryna29 sty 2024 · Cytoplasmic male sterility (CMS), which is controlled by mitochondrial genes, is an important trait for commercial hybrid seed production. So far, genes controlling this trait are still not clear in pepper. In this study, complete mitochondrial genomes were sequenced and assembled for the CMS line 138A and its maintainer …
Orfh79
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WitrynaPK Í´EU bridge/PK ïˆEU entities/PK δEU manifest.json•RMoë “_ q.Ø1† û ªêݪ§h ë 5Æ Æ}Ê‹òß‹¿Ú(·^, fvfg}Ý Zw¡…xøÄлÎÓçmö”n ... WitrynaWestern blot analysis of the specificity of the antiserum using ORFH79 expressed in E.coli A SDS-PAGE analysis of orfH79 expression in E.coli. B Western blot profile of ORFH79 protein in E.coli ...
WitrynaOne copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox II and orf107, and was special to HL-sterile … Witryna本发明公开了一种DNA分子、一种重组载体、一种细胞和一种植物。本发明还公开了一种有效防止转基因植物花粉逃逸的方法，包括如下步骤：步骤一、将DNA分子构建到表达载体上得到带有DNA分子的重组载体；步骤二、将重组载体转化到植物细胞中，筛选、培养得到含有DNA分子的转基因植株。本发明 ...
Witryna7 lis 2024 · To investigate the distribution of original CMS source, and explore the origin of gametophytic CMS gene, a total of 427 individuals with seventeen representative populations of O. rufipogon were collected in from Dongxiang of Jiangxi Province to Sanya of Hainan Province, China, for the PCR amplification of atp6, orfH79 and B-a … WitrynaTotal protein was extracts from pET-28a or pET-28a-orfH79 transformants induced with 1 mM IPTG, and subjected to PAGE in a 15 % acylamide gel. Western blot was preformed to detect the production ...
Witryna2 sie 2024 · Based on the studies on HL-type hybrid rice, the orfH79 gene expression element was synthesized by Qingke Biotechnology Co., Ltd., containing the promoter PG47, the RF1b MSP sequence and the terminator IN2-1, and was introduced into pCAMBIA1300. Then, the construct pCAMBIA1300-orfH79 was transformed into …
Witryna19 sty 2016 · If RFC3 directly binds CMS RNA, atp6-orfH79, translation of ORFH79 would be blocked by overexpression of RFC3 in sterile lines. However, overexpression of RFC3 did not rescue sterility, implying that RFC3 cannot bind CMS RNA, atp6-orfH79, and this is consistent with the role of GRP162 in the interaction between the complex … bunny ears beanie child knitted patternWitrynaAbstract. The restoration fertility complex (RFC) was previously identified in Honglian (HL)-cytoplasmic male sterility (CMS) rice (Oryza sativa), and glycine-rich protein 162 … hallex hart v colvinWitryna1 lis 2014 · In rice, ORF79 and ORFH79 were both established as cytotoxic proteins or peptides for E. coli and/or yeast (Luo et al., 2013, Peng et al., 2009, Wang et al., 2006). Furthermore, ORFH79 can be detected by BN-PAGE in HL-CMS rice and is associated with several large protein complexes ranging from 400 kDa to 1.2 MDa (Liu et al., 2012). bunny ears clipart svgWitryna30 mar 2024 · The chimeric orfH79 from CMS-HL is a variant of orf79 from CMS-BT that encodes proteins with a 5′ region similar to cox1 and a 3′ region of unknown origin [39,40]. For the rice RT98A CMS line, its orf113 has the same sequence as nad9 in the region from –151 to +11, while the rest is composed of unknown sequences [ 41 ]. bunny ears clipart imagesWitryna4 lut 2024 · Based on the alignment of amino acid sequences, four accessions with orfH79 and three accessions with orf290 were screened. Four novel CMS lines carrying orfH79 haplotypes and three novel CMS lines carrying orf290 haplotypes were then developed using multiple backcross generations with a maintainer line under MAS. … bunny ears cc sims 4Witryna28 lis 2008 · In this paper, we introduced orfH79 gene into Saccharomyces cerevisiae, an efficient eukaryotic host for heterologous gene expression, and we found that weakly … bunny ears car air freshenerWitryna小木虫,论坛,科研. （1）类风湿因子人血清中IgM、IgG型类风湿因子（RF）可以与...在用多抗测定抗原时对测定结果影响不大，但在用单克隆抗体测定抗原时，假如交叉抗原决定簇正好是所用单克隆抗体相对应的靶决定簇... hallex grant review